BabyBio™ affimAb are 1 ml and 5 ml columns prepacked with the optimized alkaline-stable resin WorkBeads affimAb designed for purification of monoclonal- and polyclonal antibodies. This resin has a superior base matrix in combination with an optimized alkaline-stable protein A ligand. This results in high dynamic binding capacity also at short residence times, and stabile capacity over multiple purification cycles with cleaning-in-place using 0.5 M NaOH.
BabyBio affimAb columns are convenient for efficient small scale purifications and initial screening in process development.
- Top performance dynamic binding capacity also at short residence times
- Outstanding alkaline stability with 0.5 M NaOH, extends the number of purification cycles
- Excellent purity, recovery and reproducibility
- Negligible protein A leakage
WorkBeads are agarose based chromatographic resins manufactured by a proprietary method that results in porous beads with a tight size distribution and very high mechanical stability. Agarose based matrices have been successfully used for decades in biotechnology research from laboratory to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity.
The alkaline stable recombinant protein A attached to the optimized basematrix is produced in E. coli under conditions free of components of animal origin and purified to high purity before coupling. This combination gives both high dynamic binding capacities for antibodies and the possibility for efficient cleaning-in-place with 0.5 M NaOH.
BabyBio column is made from biocompatible polypropylene which does not significantly interact with biomolecules. The top and bottom filters are made from low protein binding polyethylene. The ready to use BabyBio columns are delivered with a plug in the inlet, a cut-off outlet and a cap for storage. The columns can be connected to a syringe, pump or chromatography system using finger tight fittings (coned 10–32) for 1/16” o.d. tubing (standard HPLC PEEK tubing).