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IMAC resin

BabyBio IDA

BabyBio IDA

BabyBioTM IDA columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged) proteins and other proteins with an affinity for metal ions. The columns are prepacked with pre-charged or uncharged WorkBeads 40 IDA resins. They are available in two column sizes, 1 ml and 5 ml. IMAC utilizes the affinity of histidine, cysteine and tryptophan amino acid side chains on the protein surface for binding to transition metal ions, such as Ni2+, Co2+, Cu2+ and Zn2+, which are immobilized via a metal chelating ligand on the chromatography resin.

  • High binding capacity and purity
  • Pre-charged resins with different metal ions for convenient screening for optimal purity
  • Uncharged resins can be charged wih the metal ion of choice
  • Prepacked ready-to-use columns for rapid and reliable results
Product

The BabyBio IDA resins are based on the iminodiacetic acid group (IDA) chelating group. The resin can easily be charged before use with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.

 

Product numbers:
BabyBio Ni-IDA, 1 ml x 1 - 45655001
BabyBio Ni-IDA, 1 ml x 2 - 45655002
BabyBio Ni-IDA, 1 ml x 5 - 45655003
BabyBio Ni-IDA, 1 ml x 10 - 45655004
BabyBio Ni-IDA, 5 ml x 1 - 45655005
BabyBio Ni-IDA, 5 ml x 2 - 45655006
BabyBio Ni-IDA, 5 ml x 5 - 45655007
BabyBio Ni-IDA, 5 ml x 10 - 45655008

BabyBio IDA, 1 ml x 1 - 45655011
BabyBio IDA, 1 ml x 2 - 45655012
BabyBio IDA, 1 ml x 5 - 45655013
BabyBio IDA, 1 ml x 10 - 45655014
BabyBio IDA, 5 ml x 1 - 45655015
BabyBio IDA, 5 ml x 2 - 45655016
BabyBio IDA, 5 ml x 5 - 45655017
BabyBio IDA, 5 ml x 10 - 45655018

BabyBio Cu-IDA, 1 ml x 1 - 45655021
BabyBio Cu-IDA, 1 ml x 2 - 45655022
BabyBio Cu-IDA, 1 ml x 5 - 45655023
BabyBio Cu-IDA, 1 ml x 10 - 45655024
BabyBio Cu-IDA, 5 ml x 1 - 45655025
BabyBio Cu-IDA, 5 ml x 2 - 45655026
BabyBio Cu-IDA, 5 ml x 5 - 45655027
BabyBio Cu-IDA, 5 ml x 10 - 45655028

BabyBio Co-IDA, 1 ml x 1 - 45655031
BabyBio Co-IDA, 1 ml x 2 - 45655032
BabyBio Co-IDA, 1 ml x 5 - 45655033
BabyBio Co-IDA, 1 ml x 10 - 45655034
BabyBio Co-IDA, 5 ml x 1 - 45655035
BabyBio Co-IDA, 5 ml x 2 - 45655036
BabyBio Co-IDA, 5 ml x 5 - 45655037
BabyBio Co-IDA, 5 ml x 10 - 45655038

BabyBio Zn-IDA, 1 ml x 1 - 45655041
BabyBio Zn-IDA, 1 ml x 2 - 45655042
BabyBio Zn-IDA, 1 ml x 5 - 45655043
BabyBio Zn-IDA, 1 ml x 10 - 45655044
BabyBio Zn-IDA, 5 ml x 1 - 45655045
BabyBio Zn-IDA, 5 ml x 2 - 45655046
BabyBio Zn-IDA, 5 ml x 5 - 45655047
BabyBio Zn-IDA, 5 ml x 10 - 45655048

BabyBio IDA Screening kit, 1 ml - 45700001
BabyBio IDA Screening kit, 5 ml - 45700002

Applications

BabybioIDANTA-Application-1-1
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