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IMAC resin

BabyBio NTA

BabyBio NTA

BabyBio™ NTA columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged) proteins and other proteins with an affinity for metal ions. The columns are prepacked with pre-charged or uncharged WorkBeads™ 40 NTA resins. They are available in two column sizes, 1 ml and 5 ml. IMAC utilizes the affinity of histidine, cysteine and tryptophan amino acid side chains on the protein surface for binding to transition metal ions, such as Ni2+, Co2+, Cu2+ and Zn2+, which are immobilized via the metal chelating ligand on the chromatography resin.

  • High binding capacity and purity
  • Pre-charged resins with different metal ions for convenient screening for optimal purity
  • Uncharged resins can be charged wih the metal ion of choice
  • Prepacked ready-to-use columns for rapid and reliable results
Product

The BabyBio™ NTA resins are based on the nitrilotriacetic acid group (NTA) chelating group. The resin can easily be charged, before use,with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.

 

Product numbers:
BabyBio Ni-NTA, 1 ml x 1 - 45655101
BabyBio Ni-NTA, 1 ml x 2 - 45655102
BabyBio Ni-NTA, 1 ml x 5 - 45655103
BabyBio Ni-NTA, 1 ml x 10 - 45655104
BabyBio Ni-NTA, 5 ml x 1 - 45655105
BabyBio Ni-NTA, 5 ml x 2 - 45655106
BabyBio Ni-NTA, 5 ml x 5 - 45655107
BabyBio Ni-NTA, 5 ml x 10 - 45655108
BabyBio Ni-NTA, 5 ml x 100 - 45655109
BabyBio Ni-NTA, 1 ml x 100 - 45655110

BabyBio NTA, 1 ml x 1 - 45655111
BabyBio NTA, 1 ml x 2 - 45655112
BabyBio NTA, 1 ml x 5 - 45655113
BabyBio NTA, 1 ml x 10 - 45655114
BabyBio NTA, 5 ml x 1 - 45655115
BabyBio NTA, 5 ml x 2 - 45655116
BabyBio NTA, 5 ml x 5 - 45655117
BabyBio NTA, 5 ml x 10 - 45655118

BabyBio Cu-NTA, 1 ml x 1 - 45655121
BabyBio Cu-NTA, 1 ml x 2 - 45655122
BabyBio Cu-NTA, 1 ml x 5 - 45655123
BabyBio Cu-NTA, 1 ml x 10 - 45655124
BabyBio Cu-NTA, 5 ml x 1 - 45655125
BabyBio Cu-NTA, 5 ml x 2 - 45655126
BabyBio Cu-NTA, 5 ml x 5 - 45655127
BabyBio Cu-NTA, 5 ml x 10 - 45655128

BabyBio Co-NTA, 1 ml x 1 - 45655131
BabyBio Co-NTA, 1 ml x 2 - 45655132
BabyBio Co-NTA, 1 ml x 5 - 45655133
BabyBio Co-NTA, 1 ml x 10 - 45655134
BabyBio Co-NTA, 5 ml x 1 - 45655135
BabyBio Co-NTA, 5 ml x 2 - 45655136
BabyBio Co-NTA, 5 ml x 5 - 45655137
BabyBio Co-NTA, 5 ml x 10 - 45655138

BabyBio Zn-NTA, 1 ml x 1 - 45655141
BabyBio Zn-NTA, 1 ml x 2 - 45655142
BabyBio Zn-NTA, 1 ml x 5 - 45655143
BabyBio Zn-NTA, 1 ml x 10 - 45655144
BabyBio Zn-NTA, 5 ml x 1 - 45655145
BabyBio Zn-NTA, 5 ml x 2 - 45655146
BabyBio Zn-NTA, 5 ml x 5 - 45655147
BabyBio Zn-NTA, 5 ml x 10 - 45655148

BabyBio NTA Screening kit, 1 ml - 45700101
BabyBio NTA Screening kit, 5 ml - 45700102

Applications

BabybioIDANTA-Application-2
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