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IMAC resin

WorkBeads IDA

WorkBeads IDA

WorkBeads™ 40 IDA resins are based on the iminodiacetic acid (IDA) chelating groups. The resins can easily be charged, before use, with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+ and Zn2+, Ga3+ or Fe3+. They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. , which are immobilized via a metal chelating ligand on the chromatography resin.

  • Resin charged (or pre-charged) with the metal ion of choice
  • High binding capacity and purity. Outstanding flow properties.
  • Reliable and reproducible results
  • Prepacked ready-to-use columns for rapid and reliable results - see BabyBio IMAC page
Product

The WorkBeads™ IDA resins are based on the iminodiacetic acid (IDA) chelating group. The resin can easily be charged, before use,with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.

WB-IDANTA-structure

 

Product numbers:
WorkBeads 40 IDA, 25 ml - 40601001
WorkBeads 40 IDA, 150 ml - 40601003
WorkBeads 40 IDA, 1 L - 40601010
WorkBeads 40 IDA, 5 L - 40601050

WorkBeads 40 Ni-IDA, 25 ml - 40650001
WorkBeads 40 Ni-IDA, 150 ml - 40650003
WorkBeads 40 Ni-IDA, 1 L - 40650010
WorkBeads 40 Ni-IDA, 5 L - 40650050
WorkBeads 40 Ni-IDA, 10 L - 40650060


















Applications

WB-IDANTA-application-1
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