WorkBeadsTM NiMAC resin is precharged with very strongly bound nickel ions resulting in high resistance to reducing agents such as DTT and chelating substances such as EDTA. The high stability allows purification of proteins from sources such as eukaryotic cell extracts, that normally would cause significant nickel ion stripping from the resin. This reduces the need for sample pre-treatment. The resin provides high purity and binding capacity and the possibility to use high flow rates for minimized process time.
- Resin with extra strongly bound Ni2+ resulting in extremely low nickel ion leakage
- Highly resistant to reducing agents up to 20 mM DTT
- Highly resistant to chelating substances present in eukaryotic extracts, or up to 20 mM EDTA
- High purity and reproducible results