SEC resin – Desalting and buffer exchange

BabyBio Dsalt

BabyBio Dsalt

BabyBioTM Dsalt repacked, ready to use column available in 1 ml and 5 ml which allows quick, easy and convenient group separation of high and low molecular weight substances

Desalting/buffer exchange in minutes
  • Keep the activity of your sensitive proteins
  • Sample volumes from 20 μl to 7.5 ml
  • Convenient scale-up by connecting columns in series
  • Easy to use with a syringe or chromatography system

Product

Proteins and other biomolecules differ greatly in size from salts and other small molecules. Size exclusion is an efficient technique to separates the components in high molecular weight substances and low molecular weight substances. The Mr cutoff is 5 000. Buffer exchange and desalting are common uses in laboratories working with purification and analysis, BabyBio Dsalt is excellent for this.

Buffer exchange or desalting of a sample can be used to prepare for mass spectroscopy analysis, lyophilization and after certain procedures such as ion exchange chromatography. BabyBio Dsalt is a useful alternative to dialysis when larger sample volumes are used or when samples need to be processed rapidly to avoid degradation.

Applications

BabybioDsalt-Application diagrams
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