WorkBeads NTA

WorkBeads™ 40 NTA resins are based on the nitrilotriacetic acid (NTA) chelating group. The resin can easily be charged, before use, with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+ and Zn2+, Ga3+ or Fe3+. They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. , which are immobilized via a metal chelating ligand on the chromatography resin.

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  • Resin charged (or pre-charged) with the metal ion of choice
  • High binding capacity and purity. Outstanding flow properties.
  • Reliable and reproducible results
  • Prepacked ready-to-use columns for rapid and reliable results - see BabyBio IMAC page
Product

The WorkBeads™ NTA resins are based on the nitrilotriacetic acid (NTA) chelating group. The resin can easily be charged, before use,with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+ or Zn2+, Fe3+ or Ga3+.

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. The uncharged resins can also be used for divalent metal ion removal..


Applications