WorkBeads Protein A

WorkBeads™ Protein A resin is designed for purification of monoclonal- and polyclonal antibodies using affinity chromatography technique. For small scale purification and initial screening in process development we recommend BabyBio A 1 ml and 5 ml columns prepacked with WorkBeads Protein A resin. WorkBeads Protein A resin can also be used for applications in other formats, such as test tube batch adsorption, spin columns, gravity columns or multi-well filter plates. The resin can be used for immunoprecipitation experiments.

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  • High dynamic binding capacity for monoclonal and polyclonal antibodies with excellent recovery and purity
  • Stronger coupling chemistry; high pH stability and low leakage
  • Reliable, reproducible and efficient
Product

WorkBeads are agarose based chromatographic resins manufactured by a proprietary method that results in porous beads with a tight size distribution and very high mechanical stability. Agarose based matrices have been successfully used for decades in biotechnology research from laboratory to production scale, due to their exceptional compatibility with biomolecules including proteins, peptides, nucleic acids and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity. The recombinant protein A attached to the resin is developed by Medicago and produced in E. coli under conditions free of components of animal origin and purified to high purity before coupling. The protein A is engineered to facilitate an oriented coupling to the matrix. This allows high binding capacities for target proteins. The specificity of the recombinant protein A for the Fc region of IgG provides excellent purification. Each batch of protein A is tested according to stringent requirements.

The protein A ligand is coupled to the resin using a bromohydrin based method that gives high chemical stability and low ligand leakage. The high capacity, chemical stability and a well established agarose matrix make WorkBeads Protein A ideal for purification of monoclonal antibodies as well as polyclonal antibodies. For convenient small scale purifications of antibodies WorkBeads Protein A is available in BabyBio A 1 ml and BabyBio A 5 ml columns.

Applications