Documents
WorkBeads™ 40/100 SEC, WorkBeads 40/1000 SEC & WorkBeads 40/10 000 SEC are high performance size exclusion chromatography resins for laboratory and large scale separation of proteins. Made from agarose, well established and well-known in the biotech industry.
- Excellent resolution
- Robust separation results can be achieved across a wide range of proteins & conditions
- Chemically stable for cleaning in place
Product
WorkBeads™ 40 SEC resins are produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose based matrices have been successfully used over decades in biotechnology research and in the industrial purification of proteins. Agarose is proven to be excellently compatible with natural biomolecules like proteins, DNA carbohydrates etc. The material shows minimal non-specific interaction due to the hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro-pores that can contribute to local pH variations in the micro-environment in the column and distorted separations.
WorkBeads 40 SEC for Size Exclusion Chromatography has a high selectivity which means the protein peaks are well separated with greater distance from each other than comparable products made from synthetic polymers. This means that the resin has the capacity to separate proteins well, even when using high-protein loadings.
Main characteristics of WorkBeads SEC resins.
|
WorkBeads 40/100 SEC |
WorkBeads 40/1000 SEC | WorkBeads 40/10000 SEC | |
| Matrix |
Rigid, highly cross-linked agarose |
Rigid, highly cross-linked agarose | Rigid, highly cross-linked agarose |
|
Separation range¹ |
10 - 150 kD | 10 - 1200 kD | 10 - 10 000 kD |
|
Exclusion limit |
150 kD |
1200 kD | 10 000 kD |
|
Average particle size² (Dv50) |
45 µmol |
45 µmol | 45 µmol |
|
Recommended flow rate |
20 - 100 cm/h | 20 - 100 cm/h | 20 - 50 cm/h |
|
Max flow rate³˒⁴ |
300 cm/h (600 cm/h) | 300 cm/h (600 cm/h) | 300 cm/h (600 cm/h) |
|
Chemical stability |
Compatible with all standard aqueous buffers used for protein purification. Should not be stored at low pH for prolonged time. |
Compatible with all standard aqueous buffers used for protein purification. Should not be stored at low pH for prolonged time. | Compatible with all standard aqueous buffers used for protein purification. Should not be stored at low pH for prolonged time. |
|
pH stability |
2 – 13 | 2 – 13 | 2 – 13 |
| Storage in 20% ethanol | 2 to 25 °C | 2 to 25 °C | 2 to 25 °C |
¹. Globular proteins.
².The median particle size of the cumulative volume distribution.
³.15 mm (i.d.) x 900 mm column, or 25 x 200 mm (values within brackets)
⁴.Note: Make sure that the column hardware max pressure is not exceeded.