The demand for monoclonal antibodies (mAbs) continues to grow rapidly — from innovative bispecifics and Fc-fusion proteins to next-generation antibody formats. With this comes increasing pressure on downstream purification processes to deliver higher yields, shorter cycle times, and lower overall cost of goods (CoG).
At the heart of these processes lies one of the most critical steps: Protein A affinity chromatography.
WorkBeads affimAb Edge is Bio-Works’ latest generation Protein A resin, designed to meet the industry’s demand for higher productivity and robustness in antibody purification.
It combines exceptionally high dynamic binding capacity with excellent alkali stability, enabling more efficient operations and extended resin lifetime.
> 70 mg human IgG/mL resin at 6 min residence time
> 60 mg mAb/mL even at 4 min residence time
Maintains performance after repeated cleaning with 0.5 M NaOH
This means greater productivity per liter of resin, faster cycle times, and less downtime for cleaning and regeneration.
Traditional Protein A resins often struggle to balance high performance with long-term stability. The proprietary ligand chemistry and bead technology in WorkBeads affimAb Edge allow for both.
By maintaining binding capacity over many cycles of use and cleaning, users can significantly reduce resin consumption and process costs over the lifetime of a column.
Key benefits:
Lower cost per gram of purified antibody
Extended resin lifetime and reduced replacement frequency
Simplified cleaning protocols with standard NaOH solutions
Consistent performance from lab-scale to GMP-scale columns
WorkBeads affimAb Edge is available in multiple formats, supporting a seamless transition from process development and pilot scale to commercial GMP manufacturing.
Researchers can use the same resin chemistry from screening to full-scale production — ensuring reliable, reproducible results at every stage.
This scalability simplifies technology transfer and minimizes process re-validation risks, making it ideal for both in-house bioprocess development and CDMOs optimizing client projects.
As antibody formats evolve, so must purification technology.
WorkBeads affimAb Edge is not only optimized for traditional mAbs but also compatible with bispecific antibodies and Fc-fusion proteins, offering flexibility for diverse therapeutic pipelines.
By combining high capacity, robust chemical resistance, and broad applicability, it represents a step forward in Protein A chromatography — ready for modern bioprocess demands.
Interested in learning more?
Get in touch with our chromatography experts to discuss your purification setup:
Contact Bio-Works