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Trends and know-how in biotech and downstream purification.

BabyBio columns with excellent resolution and high capacities

Introduction The prepacked BabyBio™ columns for ion exchange chromatography (IEX) are designed for easy and convenient purification of protein, peptides and oligonucleotides. Giving excellent...

Purification of a 45-amino acid residue peptideusing WorkBeads 40S

Introduction Purification of biomolecules from complex feeds is often best carried out using combinations of orthogonal separation methods. We have investigated the capture of a synthetic peptide...

Increased lifetime of RPC resins in insulin production by clean-up

Introduction Recombinant DNA technology has long been used to produce therapeutic insulin and, since most of the manufacturing cost (over 90%) of insulin is associated with recovery and purification,...

Desalting of antisense oligonucleotides and other oligonucleotides

Oligonucleotides as therapeutic molecules Short oligonucleotides have become established as important molecules in therapeutics, primarily in the form of antisense oligonucleotides (ASOs), aptamers...

AIEX purification parameters for phosphorothioate oligonucleotides

Introduction Oligonucleotides with a phosphorothioate backbone Oligonucleotide (ON)-based therapeutics is an evergrowing market with an increasing number of both FDA approved ON drugs and clinical...

Properties of WorkBeads 40 IEX resins: What makes them exceptional?

Chromatography agarose resins Agarose-based chromatography resins consisting of highly cross-linked porous beads have been used for decades to purify and separate a diverse set of macromolecules....

The challenge of phosphorothioate oligonucleotidepurifications - AIEX

Introduction Since oligonucleotides (ONs) are mainly synthetically produced by solid-phase synthesis it is easy to modify them during the synthesis process. The most commonly used modification,...

What is: Ion Exchange Chromatography (IEX) ?

Introduction Ion Exchange Chromatography (IEX) is a powerful and widely used technique for separating and purifying biomolecules such as proteins, peptides, and oligonucleotides. This technique is...

Effects of elution conditions in the purification of oligonucleotides

Introduction Oligonucleotide synthesis provides custom-made oligonucleotides. The purity of oligonucleotide preparations produced by solid-phase synthesis can be very high. However, purification is...

Purification of oligonucleotides by anion exchange chromatography

Solid-phase synthesis of oligonucleotides generally give material of rather high purity. However, for many applications, and especially for therapeutics, there is a need for purification to remove...

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