The Bio-Blog

Trends and know-how in biotech and downstream purification

Interview with Slim Chiha of Prosion Therapeutics

 At the 2022 ACHEMA conference we had the pleasure of getting to talk to Slim Chiha, CEO and founder of Prosion Therapeutics who was participating in the Start-Up Challenge Award. 

Interview with Wolfgang Winter on Pharma 4.0

We talked to Wolfgang Winter, Informatics Platform R&D Director at Agilent after his very interesting talk on the current status of Pharma 4.0 at the 2022 ACHEMA conference. 

QuickTip #10: Avoiding loss of activity of Ni charged IMAC columns

Have you experienced how Nickel-charged chromatography columns used for purifying His-tagged proteins lose their blue color, become a bit yellow and can’t bind your protein anymore? It can happen...

QuickTip #9: Purifying a His-tagged protein with low expression level

Do you find yourself having to deal with a very low yield of your target protein after the expression? Are you using a His-tag? Here are some ideas to help you with your purification.

QuickTip #8: Desalting oligos for therapeutic use

If you’re purifying therapeutic oligos you’ll need to clean the desalting resins afterwards in a far higher concentration of NaOH than the usual desalting resins will tolerate. This is how to solve...

Process modelling for chromatography – how is it used?

Process modelling of chromatographic behavior, in an industrial setting, can be a useful tool in dealing with the complexities of purification process development, optimization and scale-up. It can...

QuickTip #7: Improve purity of viruses and vaccines

Changing from purification products based on synthetic polymers to agarose-based chromatography will increase the purity of the target virus.

Enhanced Capture Process - a presentation

Just before the Christmas break, Lars, Haneskog, Bio-Works R&D Director, presented a new and proven strategy for downstream processing at the New Horizons in Biologics and Bioprocessing conference....

How reversing downstream bioprocess steps can make all the difference

Improving downstream bioprocess economy depends on fine-tuning an enormous range of parameters, from culture and harvesting to purification and final polishing. Success depends on the ability to...

QuickTip #6: Use true buffers

A critical and fundamental factor in both analytical and preparative chromatography is the use of true buffers. In other words, mobile phases with buffering capacity. For example, in peptide...