Applications

Peptide purification

WorkBeads 40S is an excellent tool for purification of therapeutic peptides from crude feeds following solid-phase synthesis or after recombinant expression

Cation exchange chromatography is a useful technique for purification of many peptides. The use of cation exchange resins to purify crude feeds following solid-phase synthesis or after recombinant expression can extend the lifetime of reverse phase chromatography (RPC) resins and/or reduce the use of organic solvents in downstream purification.

WorkBeads 40S, an agarose-based cation exchange resin, has proven to be an excellent tool for purification of therapeutic peptides due to its high binding capacity and optimal flow pressure properties. WorkBeads 40S results in high purity without compromising yield. 

Download technical details and examples

Application note

Purification of peptides by cation exchange chromatography

Poster

Purification of a 45-amino acid residue peptide using WorkBeads 40S

Whitepaper

IEX and RPC compatibility in peptide purifications: a generic procedure for implementing IEX upstream of RPC

Poster

Increased lifetime of RPC resins in insulin production by clean-up using WorkBeads 40S

Application note

Optimizing the buffer conditions for a
glycopeptide conjugate API purification

Application note

Properties of WorkBeads 40 IEX resins: What makes them exceptional?