Antibody purification
WorkBeads resins, with high binding capacities and alkali stability, are ideal purification methods of monoclonal antibodies (mAbs) from screening to process scale development.
Chromatography methods for antibody purification
The expression levels of monoclonal antibodies (mAb) in biopharma production are constantly increasing and are now frequently up to 10 g/L. Efficient production therefore often requires a capture step using chromatography resins with high binding capacities over a short time and with efficient cleaning-in-place stability using NaOH up to 0.5 M. Protein A resins yield purities >90% using standard conditions without optimization, which often is enough for many lab scale purifications. Large-scale purification usually requires optimization of the washing and elution steps followed by one or more polishing purification steps using e.g. ion-exchange chromatography.
WorkBeads™ affimAb resin is an optimized alkali-stable protein A resin designed for purification of monoclonal antibodies (mAbs) from laboratory to process scale.
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Poster
Optimized purification of antibodies using a new high-capacity and alkaline stable protein A resin
Chromatography methods for antibody purification
WorkBeads resins deliver high-yield purification of monoclonal antibodies
WorkBeads affimAb
WorkBeads affimAb resin is an optimized alkali-stable protein A resin designed for the purification of monoclonal antibodies from laboratory to process scale. This resin has a superior base matrix combined with a patented, alkali-stable protein A ligand which results in high dynamic binding capacity at short residence times. The resin is designed to withstand many repeated purification cycles without losing binding capacity even when cleaning with 0.5 M NaOH between each run. For small-scale purifications and initial screening in process development, convenient prepacked GoBio Mini and GoBio Screen columns are available. For larger-scale processes, GoBio Prep and GoBio Prod columns are available in sizes up to 21L.
WorkBeads 40 TREN
Purification of antibodies from mammalian host cells, such as Chinese Hamster Ovary (CHO), results in extensive bioburden on the protein A column. Chromatins, together with host cell proteins in general, cause damage to the protein A column. Regular cleaning-in-place (CIP) is mandatory in the purification process, but accumulative fouling of the column will still occur. The maximum lifetime of the protein A column is thus an important requirement. It would be desirable to add a purification step before the protein A purification step to reduce the components that constitute the worst threat and, in the process, improve the final purity of the target antibody.
WorkBeads 40 TREN is a multimodal ion exchange chromatography (IEX) resin with a ligand (tris(2-aminoethyl)amine, TAEA) that is positively charged below pH 9. The resin has been demonstrated to be effective in the removal of impurities such as chromatin complexes (nucleic acid-protein complexes), endotoxins, and host cell proteins. We introduced WorkBeads 40 TREN upstream of WorkBeads affimAb in the flow scheme and used it in flow through mode to adsorb undesirable impurities while letting mAb pass through the column without binding.