WorkBeads affimAb, WorkBeads Protein A, WorkBeads 40 TREN

High binding capacity and high stability

Antibodies

The expression levels of monoclonal antibodies (mAb) in biopharma production are constantly increasing and are now frequently up to 10 g/L. Efficient production therefore often requires a capture step using chromatography resins with high binding capacities over a short time and with efficient cleaning-in-place stability using NaOH up to 0.5 M. Protein A resins give in general purities of >85-90% using standard conditions without optimization, which often is enough for many lab scale purifications.

Demanding large-scale purification usually requires optimization of the washing and elution steps followed by one or more polishing purification steps using e.g. ion-exchange chromatography.

WorkBeads affimAb

WorkBeads™ affimAb resin is an optimized alkali-stable resin designed for purification of monoclonal- and polyclonal antibodies in laboratory to process scale. This resin has a superior base matrix in combination with an optimized alkali-stable protein A ligand which results in high dynamic binding capacity also at short residence times and that many repeated purification cycles easily can be performed without losing binding capacity even when cleaning with 0.5 M NaOH between each run. For small scale purifications and initial screening in process development convenient prepacked BabyBio affimAb 1 ml and 5 ml columns are available.

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WorkBeads Protein A

WorkBeads Protein A is an agarose-based resin with high binding capacity and with a cleaning-in-place stability up to 10 mM NaOH. The protein A ligand is coupled to the resin using a bromohydrin-based method that gives high chemical stability and low ligand leakage. High capacity and a well-established agarose matrix make WorkBeads Protein A ideal for purification of monoclonal antibodies (mAb) as well as polyclonal antibodies.  BabyBio A 1 ml and BabyBio A 5 ml are prepacked columns with WorkBeads Protein A.

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 WorkBeads 40 TREN 

Purification of antibodies from mammalian host cells, such as Chinese Hamster Ovary (CHO), results in extensive bioburden on the protein A column. Chromatins, together with host cell proteins in general, cause damage to the protein A column. Regular cleaning-in-place (CIP) is mandatory in the purification process, but accumulative fouling of the column will still occur. Maximized lifetime of the protein A column is thus an important requirement. It would be desirable to add a purification step before the protein A purification step to reduce the components that constitute the worst threat, and in the process improve the final purity of the target antibody.

WorkBeads 40 TREN is a multimodal ion exchange chromatography (IEX) resin with a ligand (tris(2-aminoethyl)amine, TAEA) that is positively charged below pH 9. The resin has been demonstrated to be effective in removal of impurities such as chromatin complexes (nucleic acid-protein complexes), endotoxins and host cell proteins. We introduced WorkBeads 40 TREN upstream of WorkBeads affimAb in the flow scheme and used it in flow through mode to adsorb undesirable impurities while letting mAb pass through the column without binding.

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