Antibody purification
Bio-Works resins, with high binding capacities and alkali stability up to 0.5 M, are ideal for purifying monoclonal antibodies (mAbs)
The expression levels of monoclonal antibodies (mAb) in biopharma production are constantly increasing and are now frequently up to 10 g/L. Efficient production therefore often requires a capture step using chromatography resins with high binding capacities over a short time and with efficient cleaning-in-place stability using NaOH up to 0.5 M. Protein A resins yield purities >90% using standard conditions without optimization, which often is enough for many lab scale purifications. Large-scale purification usually requires optimization of the washing and elution steps followed by one or more polishing purification steps using e.g. ion-exchange chromatography.
WorkBeads™ affimAb resin is an optimized alkali-stable resin designed for purification of monoclonal antibodies (mAbs) from laboratory to process scale.


WorkBeads affimAb
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Poster
Optimized purification of antibodies using a new high-capacity and alkaline stable protein A resin
WorkBeads 40 TREN
Purification of antibodies from mammalian host cells, such as Chinese Hamster Ovary (CHO), results in extensive bioburden on the protein A column. Chromatins, together with host cell proteins in general, cause damage to the protein A column. Regular cleaning-in-place (CIP) is mandatory in the purification process, but accumulative fouling of the column will still occur. Maximized lifetime of the protein A column is thus an important requirement. It would be desirable to add a purification step before the protein A purification step to reduce the components that constitute the worst threat, and in the process improve the final purity of the target antibody.
WorkBeads 40 TREN is a multimodal ion exchange chromatography (IEX) resin with a ligand (tris(2-aminoethyl)amine, TAEA) that is positively charged below pH 9. The resin has been demonstrated to be effective in removal of impurities such as chromatin complexes (nucleic acid-protein complexes), endotoxins and host cell proteins. We introduced WorkBeads 40 TREN upstream of WorkBeads affimAb in the flow scheme and used it in flow through mode to adsorb undesirable impurities while letting mAb pass through the column without binding.