The expression levels of monoclonal antibodies (mAb) in biopharma production are constantly increasing and are now frequently up to 10 g/L. Efficient production therefore often requires a capture step using chromatography resins with high binding capacities over a short time and with efficient cleaning-in-place stability using NaOH up to 0.5 M. Protein A resins give in general purities of >85-90% using standard conditions without optimization, which often is enough for many lab scale purifications.

Demanding large-scale purification usually requires optimization of the washing and elution steps followed by one or more polishing purification steps using e.g. ion-exchange chromatography.

WorkBeads affimAb

WorkBeads™ affimAb resin is an optimized alkali-stable resin designed for purification of monoclonal- and polyclonal antibodies in laboratory to process scale. This resin has a superior base matrix in combination with an optimized alkali-stable protein A ligand which results in high dynamic binding capacity also at short residence times and that many repeated purification cycles easily can be performed without losing binding capacity even when cleaning with 0.5 M NaOH between each run. For small scale purifications and initial screening in process development convenient prepacked BabyBio affimAb 1 ml and 5 ml columns are available.

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WorkBeads affimAb for optimized purification of antibodies

Application note: Protection of protein A resins during mAb purifications

Poster: Improved key quality attributes of antibody purification processes

Poster: Efficient protection of protein A resins during mAb purifications

WorkBeads Protein A

WorkBeads Protein A is an agarose-based resin with high binding capacity and with a cleaning-in-place stability up to 10 mM. The protein A ligand is coupled to the resin using a bromohydrin-basedmethod that gives high chemical stability and low ligand leakage. High capacity and a well-established agarose matrix make WorkBeads Protein A ideal for purification of monoclonal antibodies (mAb) as well as polyclonal antibodies.  BabyBio A 1 ml and BabyBio A 5 ml are prepacked columns with WorkBeads Protein A.

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WorkBeads Protein A – chromatography resin for antibody purification

BabyBio A – convenient pre-packed columns for antibody purification