The expression levels of monoclonal antibodies (mAb) in biopharma production are frequently up to 10g/L. Efficient production is therefore often requires a capture step using chromatography resins with high binding capacities. WorkBeads Protein A is an agarose-based resin with high binding capacity and a competitive price.
Purity of >85-90% is generally obtained using standard conditions without optimization. WorkBeads Protein A thus does not require optimization for most research-scale applications. Demanding large-scale purification usually requires optimization of the washing and elution steps followed by one or more polishing purification steps using e.g. ion-exchange chromatography.
BabyBio A 1ml and BabyBio A 5ml are pre-packed columns with WorkBeads Protein A. BabyBio A columns can be used for determination of expression levels and purification in research scale, or used for method development for bioprocess.
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