Immobilized Metal Ion Affinity Chromatography (IMAC) is the chromatography technique used for purification of His-tagged proteins. It utilizes the affinity for binding of histidine to different metal ions such as Ni2+, Co2+, Zn2+ or Cu,2+ immobilized by chelating ligands on the chromatographic resin. This method is simple to use and gives very high purity in only one step. Two different WorkBeads IMAC resins are available: WorkBeads 40 IDA and WorkBeads 40 NTA. These resins can either be purchased uncharged or precharged with the different metal ions described above.
BabyBio His-tag Screening kits are available in two column sizes, 1 ml and 5 ml, pre-packed with WorkBeads 40 NTA and WorkBeads 40 IDA. These screening kits for each chelating ligand contain ready-to-use BabyBio columns, charged with four different metal ions, Ni2+, Co2+, Cu2+ and Zn2+. This diversity, combined with the excellent mechanical properties and tight size distribution of WorkBeads resin, provides the perfect starting point for purification of your His-tagged protein.
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