Recombinant protein purification
WorkBeads resins are available for all stages of protein purification, where orthogonal separation methods are often used to achieve required purity.
It is often necessary to purify proteins from complex feeds using combinations of orthogonal separation methods to achieve the required purity. The purification strategy should be planned using knowledge of the required purity of the target protein for further usage. The higher the final purity, the lower the yield. A good strategy is CEP: Capture – Enhance – Polish.
WorkBeads resins are available for all different stages. In between steps there is often a need to remove unwanted compounds and salt and to adapt conductivity and pH for the next step. This can be done in minutes using WorkBeads Dsalt. GoBio prepacked columns come prepacked with many different chromatographic resins and allow for fast and convenient start of purification without needing to pack the column.