Recombinant protein purification
WorkBeads resins are available for all stages of recombinant protein purification, where orthogonal separation methods are often used to achieve the required purity.
Chromatography methods for recombinant protein purification
It is often necessary to purify recombinant proteins from complex feeds using combinations of orthogonal separation methods to achieve the required purity. The purification strategy should be planned using knowledge of the required purity of the target protein for further usage. The higher the final purity, the lower the yield. A good strategy is CEP: Capture – Enhance – Polish.
WorkBeads resins are available for all different stages. In between steps there is often a need to remove unwanted compounds and salt and to adapt conductivity and pH for the next step. This can be done in minutes using WorkBeads Dsalt. GoBio prepacked columns come prepacked with many different chromatographic resins and allow for fast and convenient start of purification without needing to pack the column.
Download technical documents and examples
Poster
Purification of lactoferrin from low-fat bovine milk by cation-exchange chromatography
Poster
Fast and efficient screening of binding conditions for cation-exchange chromatography
WorkBeads resins deliver high-yield purification of recombinant proteins
WorkBeads Dsalt
WorkBeads Dsalt resin enables efficient desalting and/or buffer exchange of proteins, large peptides and nucleic acids. This resin can be used for buffer exchange or desalting to prepare a sample, e.g. before mass spectrometry analysis, lyophilization and before/after ion-exchange chromatography. The separation is convenient and very fast and is an excellent alternative to dialysis when samples need to be processed rapidly to avoid degradation. The chromatography format is also completely scalable up to production scale.
GoBio prepacked columns
GoBio prepacked columns are packed with WorkBeads resins. The columns are specifically designed for the purification of biological molecules such as vaccines, monoclonal antibodies, and recombinant proteins. They enable a linear scale-up from process validation to commercial and GMP manufacturing. Prepacked columns enhance facility efficiency overall by converting column packing resources into productive operations and accelerating campaign turnaround time.