Immobilized metal-ion affinity chromatography (IMAC) resin

The WorkBeads™ 40 NTA and WorkBeads 40 IDA resins are based on nitrilotriacetic acid (NTA) and the iminodiacetic acid (IDA) chelating groups. The resins can easily be charged, before use, with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+, Zn2+, Ga3+ or Fe3+. They can then be used or the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metalcharged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. These resins can also be used for divalent metal ion removal.

• Resin can be charged (or be pre-charged) with the metal ion of choice
• High binding capacity and purity. Outstanding flow properties.
• Reliable and reproducible results

© Copyright - Bio-Works Technologies AB