Applications

Oligonucleotide purification

Discover how WorkBeads 40Q anion exchange resin is a cost-effective and reproducible purification method that has a high binding capacity for producing pure oligonucleotides.

The purity of custom-made oligonucleotide preparations produced by solid-phase synthesis can be very high but purification is often required to remove incomplete or erroneous sequences. Anion exchange chromatography is an attractive choice since it can separate the negatively charged oligonucleotides based on length.

For these purifications (including siRNA and mRNA), WorkBeads 40Q has proven to be a superior chromatography resin giving high binding capacity and highly pure oligonucleotides.

Download technical documents and examples

Poster

Optimization and scale-up of oligonucleotide purifications

Application note

Purification of oligonucleotides by anion exchange chromatography

Poster

Effects of elution conditions in the purification of oligonucleotides by agarose-based anion exchange chromatography

Poster

Important optimization parameters for AIEX purifications of 45 nt-long RNA molecules

Application note

Properties of WorkBeads 40 IEX resins: What makes them exceptional?

Whitepaper

WorkBeads 40/100 SEC can be used to desalt oligonucleotides and can be cleaned with 1 M NaOH

Poster

Conversion of ion-pairing agents/counter ions for oligonucleotides using AIEX.

Application note

Optimization of AIEX purification parameters for phosphorothioate oligonucleotides

Poster

The challenge of phosphorothioate oligonucleotide purifications – optimization of AIEX parameters