The purity of custom-made oligonucleotide preparations produced by solid-phase synthesis can be very high but purification is often required to remove incomplete or erroneous sequences. Anion exchange chromatography is an attractive choice since it can separate the negatively charged oligonucleotides based on length. For these purifications WorkBeads 40Q has proven to be a superior chromatography resin giving high binding capacity and pure oligonucleotides.

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Optimization and scale-up of oligonucleotide purifications

Purification of oligonucleotides by anion exchange chromatography

Effects of elution conditions in the purification of oligonucleotides by agarose-based anion exchange chromatography