Peptide purification
WorkBeads ion exchange resins are an excellent purification solution for therapeutic peptides from crude feeds following solid-phase synthesis or after recombinant expression.
Chromatography methods for peptide purification
Cation exchange chromatography is a useful technique for purification of many peptides. The use of cation exchange resins to purify crude feeds following solid-phase synthesis or after recombinant expression can extend the lifetime of reverse phase chromatography (RPC) resins and/or reduce the use of organic solvents in downstream purification.
WorkBeads 40S, an agarose-based cation exchange resin, has proven to be an excellent tool for purification of therapeutic peptides due to its high binding capacity and optimal flow pressure properties. WorkBeads 40S results in high purity without compromising yield.
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Whitepaper
IEX and RPC compatibility in peptide purifications: a generic procedure for implementing IEX upstream of RPC
Poster
Increased lifetime of RPC resins in insulin production by clean-up using WorkBeads 40S
Application note
glycopeptide conjugate API purification
WorkBeads resins deliver high-yield purification of peptides
WorkBeads 40S
WorkBeads 40S resin is an agarose-based strong cation exchange chromatography resin (it is completely ionized over a broad pH range, 2–12) derivatized with sulfonate ligands. This high-capacity resin is manufactured using a proprietary method that results in porous beads with tight size distribution and exceptional mechanical stability. It's stable in high concentrations of sodium hydroxide, which allows efficient cleaning-in-place. WorkBeads 40S is thus an excellent choice as an upstream purification step for peptides if a cation exchange chromatography resin is preferred prior to the RPC step(s).
Cation exchange chromatography
Reverse phase chromatography (RPC) is the most commonly and frequently used peptide separation technique, but crude feeds may foul the columns. By introducing a cation exchange chromatography (CIEX) step upstream of the high-performance silica-based RPC step, the burden from impurities is significantly reduced. In addition, the purity of the target peptide is greatly enhanced.