QuickTip #5: Filter everything
Filtering is crucial in order to protect the packed column from all solid particles in feeds or eluents. Clogging of the column will increase back-pressure and disturb the feed loading zone causing...
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QuickTip #3: optimizing mAb purification
Designing an optimized purification process means maximizing yield and purity, and at the same time controlling costs. So, you remove a lot of bulk impurities during the first steps, and focus on...
QuickTip #2: choosing IEX resin
Recovery of your biologically active target substance is a priority, and it’s important to check for its pH and ionic strength stability values...
QuickTip #1: cleaning IEX columns
When an IEX resin has been used for some time, impurities may build up in the column causing loss of resolution or increase in back pressure (shown on the pressure monitor or seen by gap formation on...
How to improve process efficiency and economy in peptide production
The purity requirements for therapeutic peptides are very stringent, but synthesis generates a crude peptide mixture containing failed sequences and chemical variants, while recombinant peptides have...
Optimizing mAb purification using a guard column
What are the benefits of introducing multimodal ion exchange chromatography as a guard column and could they include a significant increase in mAb purity? This article presents a study in which two...