Blog

Trends and know-how in biotech and downstream purification.

Purification of lactoferrin from low-fat bovine milk by cation-exchange chromatography

Introduction Lactoferrin is a globular glycoprotein found in secretory fluids of mammals. It controls the concentration of free iron, and is part of the innate immune system. Several mechansism have...

Desalting and buffer exchange of protein solutions

Introduction There is often a need for removing unwanted salt or low molecular weight compounds, or to exchange the buffer in protein purification and in subsequent studies. BabyBio™ Dsalt 1 ml and...

Prepacked OptioBio columns for small-scale purifications and process development

Introduction

Fast and efficient screening of binding conditions for cation-exchange chromatography

Introduction Conductivity, pH, residence time and the pI of the protein effects the binding of proteins in ion exchange chromatography. To get the best conditions optimization is necessary. BabyBio™...

Purification of a 45-amino acid residue peptideusing WorkBeads 40S

Introduction Purification of biomolecules from complex feeds is often best carried out using combinations of orthogonal separation methods. We have investigated the capture of a synthetic peptide...

Increased lifetime of RPC resins in insulin production by clean-up

Introduction Recombinant DNA technology has long been used to produce therapeutic insulin and, since most of the manufacturing cost (over 90%) of insulin is associated with recovery and purification,...

The challenge of phosphorothioate oligonucleotidepurifications - AIEX

Introduction Since oligonucleotides (ONs) are mainly synthetically produced by solid-phase synthesis it is easy to modify them during the synthesis process. The most commonly used modification,...

Effects of elution conditions in the purification of oligonucleotides

Introduction Oligonucleotide synthesis provides custom-made oligonucleotides. The purity of oligonucleotide preparations produced by solid-phase synthesis can be very high. However, purification is...

Conversion of ion-pairing agents/counter ions for oligonucleotides using AIEX

Introduction Efficient purification of therapeutic molecules is of the utmost importance. Equally important, is the removal of residuals, such as ion-pairing agents (IP agents), used in the...

Important optimization parameters for AIEX purifications of 45 nt-long RNA molecules

Introduction RNA molecules have emerged as important molecules in therapeutics, primarily in the form of antisense oligos (ASO) and small interfering RNAs (siRNAs). Consequentially, requirements for...