The Downstream Corner

Trends, insights and expert tips from the biotech industry in general and downstream purification in particular.

QuickTip #8: Desalting oligos for therapeutic use

If you’re purifying therapeutic oligos you’ll need to clean the desalting resins afterwards in a far higher concentration of NaOH than the usual desalting resins will tolerate. This is how to solve...

QuickTip #7: Improve purity of viruses and vaccines

Changing from purification products based on synthetic polymers to agarose-based chromatography will increase the purity of the target virus.

QuickTip #5: Filter everything

Filtering is crucial in order to protect the packed column from all solid particles in feeds or eluents. Clogging of the column will increase back-pressure and disturb the feed loading zone causing...