The Downstream Corner

Trends, insights and expert tips from the biotech industry in general and downstream purification in particular.

QuickTip #5: Filter everything

Filtering is crucial in order to protect the packed column from all solid particles in feeds or eluents. Clogging of the column will increase back-pressure and disturb the feed loading zone causing...

Ten things you must know when producing synthetic therapeutic peptides

Peptide therapeutic agents are enjoying increasing success but they are challenging to produce and, not least, to purify. Purity requirements are, in fact, approaching those for small molecules....

QuickTip #4: yield of His-tagged proteins

Immobilized Metal Ion Affinity Chromatography (IMAC) is used for purification of His-tagged proteins. Ni-NTA based resins are the most commonly used as the Ni2+ ion is considered to have the...

Join the effort to make chromatography sustainable

The environmental impact of pharmaceutical R&D and production is considerable. Although the industry is rather conservative and also constrained by regulatory guidelines, there is increasing interest...

QuickTip #3: optimizing mAb purification

Designing an optimized purification process means maximizing yield and purity, and at the same time controlling costs. So, you remove a lot of bulk impurities during the first steps, and focus on...

Affordable and safe biosimilars for affordable healthcare

When it comes to many serious and challenging diseases, healthcare has reached a point of unsustainability, due to drug costs. For example, sustained therapy for an autoimmune disease or cancer,...

Are mAbs the ultimate targeted therapy?

Since the 1980’s, monoclonal antibodies (mAbs) have revolutionized healthcare and continue to do so. A short history of mAb approvals shows their increasing sophistication and targeting of disease...

QuickTip #2: choosing IEX resin

Recovery of your biologically active target substance is a priority, and it’s important to check for its pH and ionic strength stability values...

CHO cells in fed-batch bioreactors dominate mAb upstream processes

Choosing a mammalian cell line or other type of host is as critical a question as any other in the complex world of monoclonal antibody (mAb) manufacture. We take a look at the present status, with...

QuickTip #1: cleaning IEX columns

When an IEX resin has been used for some time, impurities may build up in the column causing loss of resolution or increase in back pressure (shown on the pressure monitor or seen by gap formation on...