Blog

Trends and know-how in biotech and downstream purification.

Conversion of ion-pairing agents/counter ions for oligonucleotides using AIEX

Introduction Efficient purification of therapeutic molecules is of the utmost importance. Equally important, is the removal of residuals, such as ion-pairing agents (IP agents), used in the...

A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides (2025)

The Study: A Comparative Analysis Our study, published in the Journal of Chromatography A, focused on the preparative purification of a 20-mer oligonucleotide. We systematically compared IEX and...

Important optimization parameters for AIEX purifications of 45 nt-long RNA molecules

Introduction RNA molecules have emerged as important molecules in therapeutics, primarily in the form of antisense oligos (ASO) and small interfering RNAs (siRNAs). Consequentially, requirements for...

Optimization and Scale-up of Oligonucleotide Purifications

Introduction Solid-phase synthesis of oligonucleotides generally gives material of rather high purity. However, for many applications, and especially for therapeutics, there is a need for...

Optimizing your IMAC purification

Results from IMAC purifications depend both on the chelated metal ion and the chelator itself Immobilized Metal Ion Affinity Chromatography (IMAC) utilizes the affinity of histidine, cysteine and...

Addressing the challenge of optimizing His-tagged protein purifications

Introduction Immobilized Metal Affinity Chromatography (IMAC) is the commonest technique for purification of His-tagged proteins. Tagging proteins enables the use of affinity chromatography for...

Easy optimization of His-tagged protein purification

Introduction Immobilized Metal Ion Affinity Chromatography (IMAC) utilizes the affinity of histidine, cysteine and tryptophan on the protein surface for binding to metal ions immobilized on the...

Improved key quality attributes of antibody purification processes

Introduction The rapidly growing monoclonal antibody (mAb) market and the increasing demand for improved process economy put pressure on creating efficient downstream processing. WorkBeads™ affimAb...

Advantages of investing in a custom chromatography resin for your target molecule

When it comes to optimizing and refining your purification processes, nothing can surpass the precision and effectiveness of a custom chromatography resin. It is designed for your specific...

Optimized purification of antibodies using a new high-capacity and alkaline stable protein A resin

Introduction A new protein A chromatography resin has been designed for purification of mAbs, Gabs and polyclonal antibodies. The new resin, WorkBeads™ affimAb, is designed for high dynamic binding...

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